Morphological changes in BHK-21 cells infected with S/N (H2N1) influenza virus
Identifieur interne : 002C53 ( Main/Exploration ); précédent : 002C52; suivant : 002C54Morphological changes in BHK-21 cells infected with S/N (H2N1) influenza virus
Auteurs : E. Anisimová [Tchécoslovaquie] ; E. Tu Ková [Tchécoslovaquie] ; V. Vonka [Tchécoslovaquie]Source :
- Archiv für die gesamte Virusforschung [ 0003-9012 ] ; 1973-09-01.
English descriptors
- Teeft :
- Acta virol, Blake bottles, Cell line, Cell membrane, Cell surface, Chick embryo fibroblasts, Cytoplasmic, Cytoplasmic inclusions, Cytoplasmic vacuoles, Dense inclusions, Electron microscope, Electron microscopy, Experimental virology, Fibrillar structures, Filamentous, Filamentous structures, Granular substance, Greatest number, Inclusion, Infection, Infectious virus, Influenza, Influenza virus, Intense fluorescence, Measles virus, Morphological changes, Nucleoli substance, Nucleolus, Plasma membrane, Positive cells, Present system, Smooth surface, Tissue culture cells, Vacuole, Vacuole membranes, Various size, Virology, Virus, Virus maturation, Virus particles.
Abstract
Summary: Morphological changes developing in BHK-21 cells infected with the S/N virus (a recombinant of A2/Singapore and A/NWS viruses), at the approximate m.o.i. of 1 TCD50 per cell, were studied by electron microscopy. Substantial alterations were observed in both the nucleus and cytoplasm. Changes in the nuclei were first detected eight hours after infection. The nucleoli substance was reduced and dense inclusions consisting of fibrillar structures 40–60 Å in diameter were formed. The greatest number of inclusions and their distribution over the whole nuclear area were found 24 hours after infection. Electron-dense inclusions were also detected in the cytoplasm. They were identical in shape and size with those observed in the nuclei but they consisted of fibrils 35–40 Å in diameter. These inclusions were rare eight hours after infection. Their number increased with the incubation period, reaching a maximum 24 hours after infection. Six hours after infection filamentous structures 80–90 Å in diameter were observed in the cytoplasm. It is assumed that they represent viral ribonucleoprotein. They were at first (hour 6) distributed diffusely through the whole cytoplasm and were later (hour 8–10) found mainly near the cytoplasmic membrane. Twenty four hours after the infection the filamentous structures were abundant both in the central part of the cytoplasm and in the proximity of the cellular membrane. Sometimes they formed dense agglomerations 2000–4200 Å in width. Maturation of the virion took place at the plasma membrane and in cytoplasmic vacuoles in whose proximity the filamentous structures were found. The particles either budded directly from the smooth cell membranes or from processes formed on the cell surface. Numerous rod-like particles were observed.
Url:
DOI: 10.1007/BF01250417
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001200
- to stream Istex, to step Curation: 001200
- to stream Istex, to step Checkpoint: 001798
- to stream Main, to step Merge: 002E28
- to stream Main, to step Curation: 002C53
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Morphological changes in BHK-21 cells infected with S/N (H2N1) influenza virus</title><author><name sortKey="Anisimova, E" sort="Anisimova, E" uniqKey="Anisimova E" first="E." last="Anisimová">E. Anisimová</name></author><author><name sortKey="Tu Kova, E" sort="Tu Kova, E" uniqKey="Tu Kova E" first="E." last="Tu Ková">E. Tu Ková</name></author><author><name sortKey="Vonka, V" sort="Vonka, V" uniqKey="Vonka V" first="V." last="Vonka">V. Vonka</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:4215FFBC6610C5B142D3CEA24DBE4BC63DA871A5</idno><date when="1973" year="1973">1973</date><idno type="doi">10.1007/BF01250417</idno><idno type="url">https://api.istex.fr/ark:/67375/1BB-WLJQPLW9-H/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001200</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001200</idno><idno type="wicri:Area/Istex/Curation">001200</idno><idno type="wicri:Area/Istex/Checkpoint">001798</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001798</idno><idno type="wicri:doubleKey">0003-9012:1973:Anisimova E:morphological:changes:in</idno><idno type="wicri:Area/Main/Merge">002E28</idno><idno type="wicri:Area/Main/Curation">002C53</idno><idno type="wicri:Area/Main/Exploration">002C53</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Morphological changes in BHK-21 cells infected with S/N (H2N1) influenza virus</title><author><name sortKey="Anisimova, E" sort="Anisimova, E" uniqKey="Anisimova E" first="E." last="Anisimová">E. Anisimová</name><affiliation wicri:level="3"><country xml:lang="fr">Tchécoslovaquie</country><wicri:regionArea>Department of Experimental Virology, Institute of Sera and Vaccines, Prague</wicri:regionArea><placeName><settlement type="city">Prague</settlement><region type="région" nuts="2">Bohême centrale</region></placeName></affiliation></author><author><name sortKey="Tu Kova, E" sort="Tu Kova, E" uniqKey="Tu Kova E" first="E." last="Tu Ková">E. Tu Ková</name><affiliation wicri:level="3"><country xml:lang="fr">Tchécoslovaquie</country><wicri:regionArea>Department of Experimental Virology, Institute of Sera and Vaccines, Prague</wicri:regionArea><placeName><settlement type="city">Prague</settlement><region type="région" nuts="2">Bohême centrale</region></placeName></affiliation></author><author><name sortKey="Vonka, V" sort="Vonka, V" uniqKey="Vonka V" first="V." last="Vonka">V. Vonka</name><affiliation wicri:level="3"><country xml:lang="fr">Tchécoslovaquie</country><wicri:regionArea>Department of Experimental Virology, Institute of Sera and Vaccines, Prague</wicri:regionArea><placeName><settlement type="city">Prague</settlement><region type="région" nuts="2">Bohême centrale</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Archiv für die gesamte Virusforschung</title><title level="j" type="abbrev">Archiv f Virusforschung</title><idno type="ISSN">0003-9012</idno><idno type="eISSN">1432-8798</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Vienna</pubPlace><date type="published" when="1973-09-01">1973-09-01</date><biblScope unit="volume">43</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="221">221</biblScope><biblScope unit="page" to="234">234</biblScope></imprint><idno type="ISSN">0003-9012</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0003-9012</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acta virol</term><term>Blake bottles</term><term>Cell line</term><term>Cell membrane</term><term>Cell surface</term><term>Chick embryo fibroblasts</term><term>Cytoplasmic</term><term>Cytoplasmic inclusions</term><term>Cytoplasmic vacuoles</term><term>Dense inclusions</term><term>Electron microscope</term><term>Electron microscopy</term><term>Experimental virology</term><term>Fibrillar structures</term><term>Filamentous</term><term>Filamentous structures</term><term>Granular substance</term><term>Greatest number</term><term>Inclusion</term><term>Infection</term><term>Infectious virus</term><term>Influenza</term><term>Influenza virus</term><term>Intense fluorescence</term><term>Measles virus</term><term>Morphological changes</term><term>Nucleoli substance</term><term>Nucleolus</term><term>Plasma membrane</term><term>Positive cells</term><term>Present system</term><term>Smooth surface</term><term>Tissue culture cells</term><term>Vacuole</term><term>Vacuole membranes</term><term>Various size</term><term>Virology</term><term>Virus</term><term>Virus maturation</term><term>Virus particles</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: Morphological changes developing in BHK-21 cells infected with the S/N virus (a recombinant of A2/Singapore and A/NWS viruses), at the approximate m.o.i. of 1 TCD50 per cell, were studied by electron microscopy. Substantial alterations were observed in both the nucleus and cytoplasm. Changes in the nuclei were first detected eight hours after infection. The nucleoli substance was reduced and dense inclusions consisting of fibrillar structures 40–60 Å in diameter were formed. The greatest number of inclusions and their distribution over the whole nuclear area were found 24 hours after infection. Electron-dense inclusions were also detected in the cytoplasm. They were identical in shape and size with those observed in the nuclei but they consisted of fibrils 35–40 Å in diameter. These inclusions were rare eight hours after infection. Their number increased with the incubation period, reaching a maximum 24 hours after infection. Six hours after infection filamentous structures 80–90 Å in diameter were observed in the cytoplasm. It is assumed that they represent viral ribonucleoprotein. They were at first (hour 6) distributed diffusely through the whole cytoplasm and were later (hour 8–10) found mainly near the cytoplasmic membrane. Twenty four hours after the infection the filamentous structures were abundant both in the central part of the cytoplasm and in the proximity of the cellular membrane. Sometimes they formed dense agglomerations 2000–4200 Å in width. Maturation of the virion took place at the plasma membrane and in cytoplasmic vacuoles in whose proximity the filamentous structures were found. The particles either budded directly from the smooth cell membranes or from processes formed on the cell surface. Numerous rod-like particles were observed.</div></front></TEI><affiliations><list><country><li>Tchécoslovaquie</li></country><region><li>Bohême centrale</li></region><settlement><li>Prague</li></settlement></list><tree><country name="Tchécoslovaquie"><region name="Bohême centrale"><name sortKey="Anisimova, E" sort="Anisimova, E" uniqKey="Anisimova E" first="E." last="Anisimová">E. Anisimová</name></region><name sortKey="Tu Kova, E" sort="Tu Kova, E" uniqKey="Tu Kova E" first="E." last="Tu Ková">E. Tu Ková</name><name sortKey="Vonka, V" sort="Vonka, V" uniqKey="Vonka V" first="V." last="Vonka">V. Vonka</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002C53 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002C53 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:4215FFBC6610C5B142D3CEA24DBE4BC63DA871A5
|texte= Morphological changes in BHK-21 cells infected with S/N (H2N1) influenza virus
}}
| This area was generated with Dilib version V0.6.33. |  |